Coding

Part:BBa_K3487001:Design

Designed by: Jianhua Zhou   Group: iGEM20_SZPT-CHINA   (2020-10-08)


TP-ⅠM,A polymer of Tachyplesin Ⅰ


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Characterization: By externally synthesizing an expression vector with Tachyplesin I multimers, transferring the vector into BL21 for antibiotic screening to obtain a single clone, culture it in liquid medium to OD600 and add IPTG to induce 12 hours to obtain recombinant protein, and collect After ultrafiltration and concentration, purified Tachyplesin I polymer was obtained. SDS-PAGE electrophoresis identification showed that the target protein had been obtained.



Source

References